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Microb Pathog. 1994 Jan;16(1):71-6.

Simple method of purification of Escherichia coli heat-labile enterotoxin and cholera toxin using immobilized galactose.

Author information

1
Institute for Diagnostic Reagents, Nissui Pharmaceutical Co., Ibaraki, Japan.

Abstract

A simple method for purification of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli and for purification of cholera toxin using immobilized D-galactose column is described. A single run of column chromatography yielded homogeneous toxin as demonstrated by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis.

PMID:
8057828
DOI:
10.1006/mpat.1994.1007
[Indexed for MEDLINE]

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