Format

Send to

Choose Destination
J Mol Biol. 1994 Aug 26;241(4):627-30.

Alternative splicing leads to two cholinergic proteins in Caenorhabditis elegans.

Author information

1
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104.

Abstract

The cha-1 gene of Caenorhabditis elegans encodes choline acetyl-transferase (the acetylcholine synthetic enzyme). The C. elegans unc-17 gene encodes a synaptic vesicle-associated acetylcholine transporter. The two genes thus define sequential biochemical steps in the metabolism of the neurotransmitter acetylcholine. Cloning, sequencing, and molecular analysis of the unc-17 region indicate that cha-1 and unc-17 transcripts share a 5' untranslated exon, and the rest of the unc-17 transcript is nested within the long first intron of cha-1. Thus, two proteins with related functions but with no sequences in common are produced as a result of alternative splicing of a common mRNA precursor. The structure of this transcription unit suggests a novel type of coordinate gene expression, and a temporal processing model is proposed for the regulation of cha-1 and unc-17 expression.

PMID:
8057385
DOI:
10.1006/jmbi.1994.1538
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center