Abstract
The hairless mutation of mice was caused by insertion of a murine leukemia virus. Starting with sequences flanking the provirus, a series of overlapping clones surrounding the viral integration site were obtained. By using a combination of sequencing, PCR, and exon-trapping techniques, the hairless gene was identified. It encodes a predicted protein of 1182 amino acids, including a potential zinc-finger domain. The expression patterns of the gene closely reflect the phenotype of animals carrying the hairless mutation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Exons / genetics
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Gene Expression
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In Situ Hybridization
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Leukemia Virus, Murine / genetics
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Mice
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Mice, Hairless / genetics*
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Molecular Sequence Data
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Mutagenesis, Insertional
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Mutation*
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Proteins / genetics*
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Restriction Mapping
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Sequence Analysis, DNA
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Tissue Distribution
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Transcription Factors*
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Zinc Fingers / genetics
Substances
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Proteins
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Transcription Factors
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hr protein, mouse