Format

Send to

Choose Destination
Arch Microbiol. 1994;161(6):453-9.

A cytochrome cd1-type nitrite reductase mediates the first step of denitrification in Alcaligenes eutrophus.

Author information

1
Institut für Pflanzenphysiologie und Mikrobiologie der Freien Universität Berlin, Germany.

Abstract

Respiratory nitrite reductase (NIR) has been purified from the soluble extract of denitrifying cells of Alcaligenes eutrophus strain H16 to apparent electrophoretic homogeneity. The enzyme was induced under anoxic conditions in the presence of nitrite. Purified NIR showed typical features of a cytochrome cd1-type nitrite reductase. It appeared to be a dimer of kDa subunits, its activity was only weakly inhibited by the copper chelator diethyldithiocarbamate, and spectral analysis revealed absorption maxima which were characteristic for the presence of heme c and heme d1. The isoelectric point of 8.6 was considerably higher than the pI determined for cd1 nitrite reductases from pseudomonads. Eighteen amino acids at the N-terminus of the A. eutrophus NIR, obtained by protein sequencing, showed no significant homology to the N-terminal region of nitrite reductases from Pseudomonas stutzeri and Pseudomonas aeruginosa.

PMID:
8048839
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center