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Crit Care Med. 1994 Aug;22(8):1211-8.

Effect of a recombinant endotoxin-neutralizing protein on endotoxin shock in rabbits.

Author information

1
Department of Medicine, Children's Hospital, Harvard Medical School, Boston, MA 02115.

Abstract

OBJECTIVES:

Limulus anti-lipopolysaccharide factor, an 11.8-kilodalton peptide isolated from amebocytes of Limulus polyphemus inhibits the biologic activities of endotoxin in vitro, including gelation of Limulus amebocyte lysate. A recombinant version of Limulus anti-lipopolysaccharide factor, termed endotoxin neutralizing protein, has now been expressed in yeast. Endotoxin-neutralizing protein was evaluated for its potential prophylactic and therapeutic effects in rabbits challenged with Escherichia coli endotoxin.

DESIGN:

Controlled animal trial.

SETTING:

Animal research laboratory.

SUBJECTS:

A total of 112 New Zealand white rabbits were studied.

INTERVENTIONS:

Rabbits were challenged with an LD80 dose of E. coli endotoxin (100 micrograms/kg); control animals (n = 52) were treated with saline solution at the time of endotoxin challenge; experimental animals received endotoxin-neutralizing protein 2.5 mg/kg prechallenge (n = 20), 5.0 mg/kg prechallenge (n = 20), or 5.0 mg/kg 30 mins postchallenge (n = 20).

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[Indexed for MEDLINE]

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