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Gene. 1994 Jun 24;144(1):147-8.

Cloning and characterization of the pho-2+ gene encoding a repressible alkaline phosphatase in Neurospora crassa.

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Biomolecular Chemistry Department, University of Wisconsin, Madison 53706.


The Neurospora crassa phosphate-repressible alkaline phosphatase-encoding gene pho-2+ was cloned and its nucleotide sequence was determined. An open reading frame was found that contains four introns and encodes a putative protein of 555 amino acids. 'Activator-independent expression' of ectopically integrated pho-2+ was observed, as noted before for ectopically integrated pho-4+.

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