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Pept Res. 1994 Jan-Feb;7(1):20-3.

Immunization and affinity purification of antibodies using resin-immobilized lysine-branched synthetic peptides.

Author information

1
Max-Planck-Institut für Immunobiologie, Germany.

Abstract

A new method has been developed to raise antibodies against synthetic peptides. A multiple antigenic peptide system (MAP) containing a branched oligolysine was synthesized on a beaded polystyrene polyoxyethylene graft copolymer resin, which acts as a synthetic hapten carrier for use in immunization. The peptides, already attached to the carrier, can be used directly after final deprotection without any further purification steps. The utility of this peptide-carrier conjugate is highlighted by its additional application for affinity purification of antibodies generated.

PMID:
8019057
[Indexed for MEDLINE]

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