A simplified method for large-scale HPA-1a phenotyping for antenatal screening

P Metcalfe; H A Doughty; M F Murphy; A H Waters

doi:10.1111/j.1365-3148.1994.tb00239.x

A simplified method for large-scale HPA-1a phenotyping for antenatal screening

Transfus Med. 1994 Mar;4(1):21-4. doi: 10.1111/j.1365-3148.1994.tb00239.x.

Authors

P Metcalfe¹, H A Doughty, M F Murphy, A H Waters

Affiliation

¹ Department of Haematology, St Bartholomew's Hospital and Medical College, London, U.K.

PMID: 8012489
DOI: 10.1111/j.1365-3148.1994.tb00239.x

Abstract

A simplified method for large-scale HPA-1a phenotyping of platelets was developed for use in an antenatal screening programme for fetomaternal alloimmune thrombocytopenia (FMAIT). The test was based on the MAIPA assay, which was modified for antigen-typing with a well-characterized anti-HPA-1a reagent. The resulting assay gave reliable results, was inexpensive and allowed testing of large batches using semiautomated equipment.

MeSH terms

Antibodies, Monoclonal / immunology
Antigens, Human Platelet / analysis*
Antigens, Human Platelet / genetics
Databases, Factual
Female
Genetic Testing* / economics
Humans
Immunoenzyme Techniques
Integrin beta3
Phenotype
Pregnancy / blood*
Pregnancy / immunology
Serology
Thrombocytopenia / genetics
Thrombocytopenia / immunology
Thrombocytopenia / prevention & control*

Substances

Antibodies, Monoclonal
Antigens, Human Platelet
ITGB3 protein, human
Integrin beta3