Format

Send to

Choose Destination
Avian Dis. 1994 Jan-Mar;38(1):50-8.

Bordetella avium hemagglutination and motility mutants: isolation, characterization, and pathogenicity.

Author information

1
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.

Abstract

Transposon mutagenesis was used to produce Bordetella avium mutants, which were screened for the lack of potential virulence factors, including a hemagglutinin, flagella, pili, and toxins. A mini-Tn10 transposon containing a kanamycin-resistance gene was introduced into the chromosomal DNA of the virulent 002/S isolate by electroporation. A hemagglutination-negative (HA-) mutant and a motility-negative mutant were obtained. Southern blot analysis showed that only the motility-negative mutant contained the transposon, whereas the HA- mutant was a spontaneous kanamycin-resistant mutant. Both mutants were stable in vitro and in vivo. Following inoculation of 2-week-old poults, the HA- mutant was determined to be less virulent than the 002/S parent, whereas the motility-negative mutant was similar in virulence to the 002/S parent. These results indicate that the hemagglutinin of B. avium is a virulence factor, but motility does not appear to contribute to virulence.

PMID:
8002900
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center