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Mol Microbiol. 1994 Aug;13(4):745-53.

Galactose-binding site in Escherichia coli heat-labile enterotoxin (LT) and cholera toxin (CT).

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1
Department of Biological Structure SM-20, University of Washington, Seattle 98195.

Abstract

The galactose-binding site in cholera toxin and the closely related heat-labile enterotoxin (LT) from Escherichia coli is an attractive target for the rational design of potential anti-cholera drugs. In this paper we analyse the molecular structure of this binding site as seen in several crystal structures, including that of an LT:galactose complex which we report here at 2.2 A resolution. The binding surface on the free toxin contains several tightly associated water molecules and a relatively flexible loop consisting of residues 51-60 of the B subunit. During receptor binding this loop becomes tightly ordered by forming hydrogen bonds jointly to the GM1 pentasaccharide and to a set of water molecules which stabilize the toxin:receptor complex.

[Indexed for MEDLINE]

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