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J Basic Microbiol. 1994;34(5):317-22.

Characterization of a Tn925-induced mutant of Staphylococcus aureus altered in exoprotein production.

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Departmento de Microbiología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional Río Cuarto, Córdoba.


An insertional mutant was isolated from a bovine Staphylococcus aureus strain after membrane-mating with a Streptococcus faecalis strain carrying conjugative plasmid pCF10::Tn925. This mutant, designated RC128, showed enhanced production of alpha-hemolysin and proteases and decreased production of coagulase, extracellular protein A, DNase, lipase and delta-hemolysin. No difference was found in the production of beta-hemolysin. Both, Southern blot analysis and transfer of the pleiotropic mutant phenotype by transduction, indicated that the mutation was originated from a single insertion of transposon Tn925. The LD50 determined by intraperitoneal administration in mice showed that mutant RC128 was slightly less virulent than its parental strain.

[Indexed for MEDLINE]

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