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C R Acad Sci III. 1994 Feb;317(2):141-8.

Multiple group II self-splicing introns in mobile DNA from Escherichia coli.

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Centre de Génétique Moléculaire du CNRS, Laboratoire associé à l'Université Pierre-et-Marie-Curie, Gif-sur-Yvette, France.


By PCR (polymerase chain reaction) amplification and cloning, we have identified four group II self-splicing introns encoding proteins related to reverse transcriptases in natural Escherichia coli isolates belonging to the ECOR collection. One intron, IntD, interrupts a DNA sequence virtually identical to that of the previously described IS3411 Insertion Sequence. A second intron, IntC, is located within an open reading frame that is closely related to a reading frame in the T-DNA of Agrobacterium tumefaciens. Finally, introns IntA and IntB are inserted at two distinct sites in one of the Rhs elements of E. coli. A comparison of their open reading frames shows that the two Rhs introns are more closely related to each other than to any other known group II intron: this suggests that transposition of group II introns may occur preferentially in cis, along the same piece of DNA.

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