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FEMS Microbiol Lett. 1994 Oct 15;123(1-2):179-84.

Morphology of the viable but nonculturable Vibrio cholerae as determined by the freeze fixation technique.

Author information

1
Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Abstract

The morphology of the nonculturable Vibrio cholerae strain TSI-4 was examined by the freeze fixation technique of electron microscopy and subsequently four unique structures were found in the fine structure s of this bacterium. The size of the cell was about 2/3 of the growing cell. Although the cell was observed to have an outer membrane as well as the cell membrane and cytoplasm, the outer membrane was undulated and had a surface layer of fine fibers. The peptidoglycan layer was thick and more electron dense than that of normal cells.

[Indexed for MEDLINE]

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