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Arch Biochem Biophys. 1994 Nov 15;315(1):54-9.

The translation efficiency of ovalbumin mRNA is determined in part by a 5'-end hairpin structure.

Author information

1
Department of Biochemistry, University of Tennessee, Memphis 38163.

Abstract

We have investigated the role in translation of the secondary structure in the noncoding leader (NCL) sequence of ovalbumin mRNA. Deletion of a stem-loop structure from the mRNA 5'-end (hairpin-1) produced a 2.5-fold decrease in mRNA translation rate in both rabbit reticulocyte wheat germ cell-free systems. A corresponding 2-fold reduction in mRNA binding affinity for reticulocyte eucaryotic initiation factor 2 (eIF-2) was also observed. These effects were independent of mRNA capping. Both translation rate and eIF-2 binding affinity were restored by addition to the mRNA of a sequence containing a hairpin-1-like structure. The positive correlation between cell free translation rate and mRNA binding to eIF-2 suggests that hairpin-1 is both an initiation signal and part of a specific eIF-2 recognition site. Methylation interference indicated a direct interaction between eIF-2 and hairpin-1 of ovalbumin mRNA.

PMID:
7979405
DOI:
10.1006/abbi.1994.1470
[Indexed for MEDLINE]

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