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Anal Biochem. 1994 Aug 1;220(2):360-6.

Iodixanol: a nonionic iso-osmotic centrifugation medium for the formation of self-generated gradients.

Author information

1
Department of Biology, University of Essex, Colchester, United Kingdom.

Abstract

The physical and biological properties of Iodixanol, a new nonionic density gradient medium, are described in this paper. It is effectively a dimer of Nycodenz and it exhibits two significant advantages over previous iodinated density gradient media--its aqueous solutions are iso-osmotic up to a density of 1.32 g/ml and it is capable of forming self-generating gradients in 1 to 3 h. It has a very low toxicity toward biological material and enzyme assays can be carried out in its presence.

PMID:
7978279
DOI:
10.1006/abio.1994.1350
[Indexed for MEDLINE]

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