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Anal Biochem. 1994 Aug 1;220(2):321-3.

Isolation and quantitation of long-chain acyl-coenzyme A esters in brain tissue by solid-phase extraction.

Author information

1
Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, Maryland 20892.

Abstract

Long-chain acyl-CoA's are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and determination of acyl-CoA's. The analysis includes solid-phase extraction by an oligonucleotide purification cartridge and HPLC measurements using a synthetic internal standard. Estimates of concentration in rat brain are oleoyl-CoA (11.0 nmol/g), palmitoyl-CoA (6.0 nmol/g), stearoyl-CoA (4.0 nmol/g), and linoleoyl- and arachidonoyl-CoA (2.0 nmol/g) for a total concentration of 23 nmol/g brain.

PMID:
7978274
DOI:
10.1006/abio.1994.1344
[Indexed for MEDLINE]

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