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Mol Cell Biol. 1994 Dec;14(12):7884-90.

Replication factor A is required in vivo for DNA replication, repair, and recombination.

Author information

1
Dipartimento di Genetica e di Biologia dei Microrganismi, Università di Milano, Italy.

Abstract

Replication factor A (RF-A) is a heterotrimeric single-stranded-DNA-binding protein which is conserved in all eukaryotes. Since the availability of conditional mutants is an essential step to define functions and interactions of RF-A in vivo, we have produced and characterized mutations in the RFA1 gene, encoding the p70 subunit of the complex in Saccharomyces cerevisiae. This analysis provides the first in vivo evidence that RF-A function is critical not only for DNA replication but also for efficient DNA repair and recombination. Moreover, genetic evidence indicate that p70 interacts both with the DNA polymerase alpha-primase complex and with DNA polymerase delta.

PMID:
7969128
PMCID:
PMC359327
[Indexed for MEDLINE]
Free PMC Article

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