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Genome. 1994 Aug;37(4):550-5.

Polymerase chain reaction mediated localization of RFLP clones to microisolated translocation chromosomes of barley.

Author information

1
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.

Abstract

A new strategy has been devised and used for the physical localization of genetically mapped restriction fragment length polymorphism (RFLP) clones to barley chromosomes. Morphologically distinct translocation chromosomes from synchronized root-tip meristems were microisolated and their DNA was used as a template for polymerase chain reaction with sequence-specific primers. Four RFLP clones were assigned to cytologically defined segments of chromosome 5. This related approximately one-third of the map length of linkage group 5 to approximately one-fifth of the mitotic metaphase length of chromosome 5. The technique may substantially contribute to the connection of the RFLP-based genetic linkage maps with cytological markers of the barley chromosomes.

PMID:
7958821
DOI:
10.1139/g94-078
[Indexed for MEDLINE]

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