Format

Send to

Choose Destination
FEBS Lett. 1994 Nov 21;355(1):41-4.

Inositol phospholipid metabolism in Xenopus oocytes mediated by endogenous G(o) and Gi proteins.

Author information

1
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.

Abstract

To characterize G-proteins which mediate the signal transduction from ligand stimulated receptor to phospholipase C (PLC), we injected antisense DNAs complementary to Xenopus G(o) alpha or Gi-l alpha to suppress these endogenous G-proteins, together with the mRNAs encoding metabotropic glutamate receptor 1 (mGluR1), 5 (mGluR5) or with M1 type muscarinic receptor into oocytes. Receptor-stimulated chloride current responses were reduced by the suppression of Xenopus G(o) alpha regardless of the types of receptors. However, injection of Gi-1 antisense DNA resulted in the reduction of M1-stimulated responses but not mGluR-stimulated responses. These results suggested that all these receptors could use G(o) alpha, and M1 receptors, but not mGluRs, could also use Gi-1 proteins, to activate PLC in Xenopus oocytes.

PMID:
7957959
DOI:
10.1016/0014-5793(94)01170-2
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center