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Exp Cell Res. 1994 Nov;215(1):145-53.

Formation of thick, curving bundles of actin by drebrin A expressed in fibroblasts.

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Department of Neurobiology and Behaviour, Gunma University School of Medicine, Maebashi, Japan.


Drebrin A is a neuron-specific protein, the expression of which is regulated during development. Upon transfection of fibroblasts with drebrin A cDNA, the protein is expressed at high levels in fibroblasts and the outgrowth of highly branched, neurite-like cell processes is induced. In this report, we describe a biochemical examination of the binding of drebrin A to actin filaments. We also demonstrate by an immunocytochemical method that, when drebrin A is expressed in transfected cells, it binds to actin filaments and is concentrated in cell processes. Furthermore, we provide evidence that thick, curving bundles of actin together with drebrin are formed in some of the transfected cells. Our results suggest that the actin filaments that bind drebrin might be a novel class of actin filaments and might play a role in neuronal morphogenesis.

[Indexed for MEDLINE]

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