Matrix-assisted laser desorption/ionization mass spectrometry of restriction enzyme-digested plasmid DNA using an active Nafion substrate

Rapid Commun Mass Spectrom. 1994 Sep;8(9):687-91. doi: 10.1002/rcm.1290080904.

Abstract

Matrix-assisted laser desorption/ionization using a 3-hydroxypicolinic acid matrix from an active Nafion substrate has been used for detection of restriction enzyme-digested double-stranded plasmid DNA using time-of-flight mass spectrometry. DNA strands of up to 267 base pairs were detected with minimal sample purification, although only as species corresponding to single-stranded DNA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA / chemistry*
  • DNA / isolation & purification
  • DNA Restriction Enzymes / chemistry
  • Fluorocarbon Polymers / chemistry*
  • Humans
  • Lasers
  • Mass Spectrometry
  • Molecular Sequence Data
  • Picolinic Acids
  • Plasmids / chemistry*
  • Polymerase Chain Reaction

Substances

  • Fluorocarbon Polymers
  • Picolinic Acids
  • perfluorosulfonic acid
  • 3-hydroxypicolinic acid
  • DNA
  • DNA Restriction Enzymes