Send to

Choose Destination
Plant Mol Biol. 1994 Oct;26(1):353-62.

Arabidopsis heat shock factor: isolation and characterization of the gene and the recombinant protein.

Author information

Universität Tübingen, Biologisches Institut, Germany.


We have isolated the Arabidopsis heat shock factor gene Athsf1 as genomic and corresponding cDNA sequences via cross-hybridization with tomato clones. Sequence analysis indicates only a partial homology with the HSFs from tomato and other organisms which is confined to the DNA-binding and the oligomerization domains. The gene is constitutively expressed but the level of mRNA for Athsf1 increases two-fold upon heat shock. However, the putative promoter region lacks the canonical heat shock elements. After expression in Escherichia coli the recombinant Athsf1 protein binds specifically to a synthetic oligonucleotide containing five heat shock elements. The native size of recombinant ATHSF1 in vitro is consistent with a trimer as demonstrated by chemical cross-linking and pore exclusion limit analysis.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center