(A) A wild-type sample showing dlg staining at type Ib boutons (long arrow) at muscles 6 7, 12, and 13, and type III boutons at muscle 12 (short arrow).
(B) A dlgm52/Df sample showing muscles 6, 7, 12, and 13, which are almost devoid of immunoreactivity at type Ib boutons. Note that immunoreactivity at type III boutons is not affected in the mutant. Image acquisition and processing parameters were identical for wild-type and mutant samples.
(C) Western blot of body wall muscles of wild-type and dlg mutants. Each lane was loaded with 2 µg of protein. Lane 1, wild type; lane 2, dlgv59/Df; lane 3, dlgm52/Df; lane 4, dlgm30/Df. Molecular masses of intensely stained bands are indicated to the right in kilodaltons. Scale bar equals 50 µ in (A) and 80 µm in (B).