Format

Send to

Choose Destination
Nucleic Acids Res. 1994 Sep 11;22(18):3801-5.

A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods.

Author information

1
Microbial Pathogenicity Group, School of Biology and Biochemistry, University of Bath, UK.

Abstract

Taguchi methods are used widely as the basis for development trials during industrial process design. Here, we describe their suitability for optimisation of the PCR. Unlike conventional strategies, these arrays revealed the effects and interactions of specific reaction components simultaneously using just a few reactions, negating the need for extensive experimental investigation. Reaction components which effected product yield were easily determined. In addition, this technique was applied to the qualitative investigation of RAPD-PCR profiles, where optimisation of the size and distribution of a number of products was determined.

PMID:
7937094
PMCID:
PMC308365
DOI:
10.1093/nar/22.18.3801
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center