Abstract
The rat HBP1 cDNA was cloned by its capacity to suppress the potassium transport-defective phenotype of mutant Saccharomyces cerevisiae cells. HBP1 cDNA encodes a 513 amino acids protein which, unexpectedly, does not share any homology with K+ transporters or K+ channels. However, a search in protein databases reveals that HBP1 contains a putative DNA-binding domain called HMG-box. Northern blot analysis shows that HBP1 is expressed in a variety of tissues and that in adipocyte and myogenic cell lines, its expression is directly related to differentiation. Taken together, the results suggest that the rat HBP1 is a new member of the HMG class of transcriptional regulators involved in cell differentiation pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipocytes / cytology
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Amino Acid Sequence
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Animals
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Brain Chemistry
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Cell Differentiation
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Cell Line
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Cloning, Molecular
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DNA, Complementary
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Gene Expression
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Gene Library
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HMGB1 Protein
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High Mobility Group Proteins / genetics*
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High Mobility Group Proteins / physiology
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Ion Transport
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Molecular Sequence Data
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Muscles / cytology
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Organ Specificity
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Potassium / metabolism*
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RNA, Messenger / biosynthesis
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Rats
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Repressor Proteins*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Sequence Analysis, DNA
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Transcription Factors / genetics*
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Transcription Factors / physiology
Substances
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DNA, Complementary
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HMGB1 Protein
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Hbp1 protein, rat
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High Mobility Group Proteins
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RNA, Messenger
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Repressor Proteins
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Transcription Factors
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Potassium