Format

Send to

Choose Destination
Mol Microbiol. 1993 Nov;10(4):799-812.

Mode and origin of replication of pSAM2, a conjugative integrating element of Streptomyces ambofaciens.

Author information

1
Institut de Génétique et Microbiologie, URA CNRS 1354, Bâtiment 400, Université Paris-Sud, Orsay, France.

Abstract

pSAM2 is an 11 kb integrating element from Streptomyces ambofaciens that is capable of replication. It generates single-stranded DNA during replication, and is therefore the first Streptomyces integrating element to be described that may belong to the family of elements, called the ssDNA elements, that replicate by a rolling-circle mechanism. The direction of replication has been identified. The plus origin (ori) of replication and minus origin (M-O) have been located. Streptomyces lividans harbouring replicating pSAM2 also contain numerous small covalently closed circular DNA molecules (scm) derived from pSAM2. These scm contain ori and extend on both sides of the putative nick site. Sequences at the junction points of these scm are heterogeneous but short direct repeats were always found in the vicinity of these junctions.

PMID:
7934842
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center