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J Cell Physiol. 1994 Oct;161(1):71-6.

Profile of energy metabolism in a murine hybridoma: glucose and glutamine utilization.

Author information

1
Department of Microbiology, University of Manitoba, Winnipeg, Canada.

Abstract

The antibody-secreting murine hybridoma, CC9C10, was grown in batch culture in a medium containing 20 mM glucose and 2 mM glutamine. After 2 days of exponential growth, the glutamine content of the medium was completely depleted, whereas the glucose content was reduced to 60% of the original concentration. The glucose and glutamine metabolism was analyzed at midexponential phase by use of radioactively labelled substrates. Glycolysis accounted for the metabolism of most of the glucose utilized (> 96%) with flux through the pentose phosphate pathway (3.6%) and the TCA cycle (0.6%) accounting for the remainder. Glutamine was partially oxidised via glutaminolysis to alanine (55%), aspartate (3%), glutamate (4%), lactate (9%), and CO2 (22%). Calculation of the theoretical ATP production from these pathways indicated that glucose could provide 59% and glutamine 41% of the energy requirement of the cells.

PMID:
7929610
DOI:
10.1002/jcp.1041610110
[Indexed for MEDLINE]

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