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Gene. 1994 Sep 30;147(2):223-6.

pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos.

Author information

1
Department of Zoology, University of Texas at Austin 78712.

Abstract

We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1 alpha-encoding gene (EF-1 alpha). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.

PMID:
7926804
DOI:
10.1016/0378-1119(94)90070-1
[Indexed for MEDLINE]

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