Structure of the gene for human uracil-DNA glycosylase and analysis of the promoter function

FEBS Lett. 1994 Oct 17;353(2):180-4. doi: 10.1016/0014-5793(94)01042-0.

Abstract

The gene for human uracil-DNA glycosylase (UNG) contains 4 exons and has an approximate size of 13 kb. The promoter is very GC rich and lacks a TATA box. Nested deletions of the promoter demonstrated that two SP1 elements and a putative c-MYC element proximal to the transcription initiation region were sufficient to support some 27% of the promoter activity, while a clone that in addition contained the elements E2F/SP1/CCAAT increased expression to almost 90% of the full-length construct. A region upstream of these elements appears to exert a negative control function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • Blotting, Southern
  • DNA / chemistry*
  • DNA Glycosylases*
  • DNA Restriction Enzymes
  • Exons
  • Humans
  • Molecular Sequence Data
  • N-Glycosyl Hydrolases / genetics*
  • Promoter Regions, Genetic*
  • Proto-Oncogene Proteins c-myc / metabolism
  • Restriction Mapping
  • Sp1 Transcription Factor / metabolism
  • TATA Box
  • Transcription Factors / metabolism
  • Uracil-DNA Glycosidase

Substances

  • Proto-Oncogene Proteins c-myc
  • Sp1 Transcription Factor
  • Transcription Factors
  • DNA
  • DNA Restriction Enzymes
  • DNA Glycosylases
  • N-Glycosyl Hydrolases
  • Uracil-DNA Glycosidase

Associated data

  • GENBANK/X79093