Format

Send to

Choose Destination
See comment in PubMed Commons below
J Neurosci Res. 1994 Mar 1;37(4):466-74.

Differential expression of Lewis(x) and sialyl-Lewis(x) antigens in fetal human neural cells in culture.

Author information

1
Department of Medicine, University of British Columbia, Vancouver, Canada.

Abstract

Lewis(x) is a cell-surface carbohydrate antigen defined by the trisaccharide structure, Gal beta 1-->4 (Fuc alpha 1-->3) GlcNAc. Expression of Lewis(x) and sialyl-Lewis(x) antigens in primary cell cultures isolated from fetal human brains of 12-15 weeks gestation was investigated by double immunolabelling with antibodies against monomeric Lewis(x) (4C9), oligomeric Lewis(x) (FH4), and sialylated oligomeric Lewis(x) (FH6) antigens and cell type-specific markers. The monomeric Lewis(x) antigen was expressed in more than 15% of astrocytes and 100% of oligodendrocytes, whereas it was not identified in neurons or in microglia. The oligomeric Lewis(x) antigen was undetectable in any cell types, while the sialylated oligomeric Lewis(x) antigen was expressed in more than 95% of microglia but not in any other cell types. The cell type-specific expression of Lewis(x) and sialyl-Lewis(x) antigens in fetal human glial cells suggests that these fucose-containing carbohydrate molecules play roles in intercellular recognition between distinct cell types during the development of the human central nervous system.

PMID:
7912739
DOI:
10.1002/jnr.490370406
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center