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FEMS Microbiol Lett. 1994 Feb 1;116(1):19-24.

Restriction fragment length polymorphisms of 16S rRNA genes in the differentiation of fast-growing mycobacterial species.

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Departamento de Medicina Preventiva, Facultad de Medicina, Universidad Autonoma, Madrid, Spain.


DNA from several species of fast growing mycobacteria displayed a characteristic restriction fragment length polymorphism (RFLP) pattern when hybridizated to a Mycobacterium fortuitum 16S rRNA gene fragment. The resulting patterns were identical when comparing different strains belonging to the same species. The RFLP results were consistent with those obtained by DNA-DNA hybridization studies. Using this approach, we have been able to identify the number of copies for 16S rRNA genes in several fast-growing mycobacteria.

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