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Plant Cell. 1994 Jan;6(1):107-18.

A novel AT-rich DNA binding protein that combines an HMG I-like DNA binding domain with a putative transcription domain.

Author information

1
Department of Biology, New York University, New York 10003.

Abstract

There is growing evidence that AT-rich promoter elements play a role in transcription of plant genes. For the promoter of the nuclear gene for chloroplast glutamine synthetase from pea (GS2), the deletion of a 33-bp AT-rich sequence (box 1 native) from the 5' end of a GS2 promoter-beta-glucuronidase (GUS) fusion resulted in a 10-fold reduction in GUS activity. The box 1 native element was used in gel shift analysis and two distinct complexes were detected. One complex is related to the low-mobility complex reported previously for AT-rich elements from several other plant promoters. A multimer of the box 1 sequence was used to isolate a cDNA encoding an AT-rich DNA binding protein (ATBP-1). ATBP-1 is not a high-mobility group protein, but it is a novel protein that combines a high-mobility group I/Y-like DNA binding domain with a glutamine-rich putative transcriptional domain.

PMID:
7907505
PMCID:
PMC160420
DOI:
10.1105/tpc.6.1.107
[Indexed for MEDLINE]
Free PMC Article

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