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Brain Res. 1993 Nov 12;627(2):299-306.

L-glutamate excitation of A10 dopamine neurons is preferentially mediated by activation of NMDA receptors: extra- and intracellular electrophysiological studies in brain slices.

Author information

1
Department of Pharmacology, College of Medicine, University of Arizona, Tucson 85724.

Abstract

The aim of the present study was to assess the effects of L-glutamate (L-GLU) on the neurophysiology of ventral tegmental A10 dopamine neurons in rat midbrain slices using extracellular and intracellular recording methods. L-Glutamate perfusion of 10-100 microM concentrations produced dose-dependent increases in firing rate, with no changes in pattern of firing, while higher concentrations led to a loss of activity reminiscent of depolarization inactivation. The extracellular changes were reflected by the pronounced membrane depolarizations observed through intracellular recordings. The effects of low doses (< or = 30 microM) of L-GLU on firing rate and membrane potential were completely antagonized by co-perfusion with the noncompetitive NMDA blocker, phencyclidine, or the selective competitive NMDA receptor antagonist, CGS 19755, but not by the selective non-NMDA blocker NBQX. However, at concentrations of > or = 300 microM L-GLU's effects could not be completely blocked without the presence of both CGS 19755 and NBQX. Moreover, the magnitude of L-GLU-induced depolarizations became attenuated at membrane potentials more negative than -70 mV. These results suggest that in physiological-like conditions that low extracellular levels of glutamate excite midbrain dopamine neurons via a preferential activation of NMDA receptors, and that only at higher concentrations of L-GLU are non-NMDA receptors brought into play.

PMID:
7905352
DOI:
10.1016/0006-8993(93)90334-j
[Indexed for MEDLINE]

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