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Appl Biochem Biotechnol. 1993 Aug-Sep;42(2-3):189-200.

DNA amplification fingerprinting using arbitrary oligonucleotide primers.

Author information

1
Institute of Agriculture, University of Tennessee, Knoxville 37901-1071.

Abstract

DNA amplification fingerprinting (DAF) is a strategy for genetic typing and mapping that uses one or more very short (> or = 5 nt) arbitrary oligonucleotides to direct the enzymatic amplification of discrete portions of a DNA template resulting in a spectrum of products characteristic of the DNA starting material. Polymorphisms from simple banding patterns are useful as genetic markers while more complex and informative patterns are suitable for DNA fingerprinting. The use of polyacrylamide gel electrophoresis and silver staining can adequately resolve the spectrum of DAF products into detailed and reproducible patterns.

PMID:
7902694
DOI:
10.1007/bf02788052
[Indexed for MEDLINE]

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