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Diagn Microbiol Infect Dis. 1993 Aug-Sep;17(2):129-33.

Restriction enzyme fragment length polymorphisms of amplified herpes simplex virus type-1 DNA provide epidemiologic information.

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Department of Pathology, University of Iowa College of Medicine, Iowa City 52242.


Human herpes simplex type 1 (HSV-1) DNA of isolates from patients in a large teaching hospital was amplified by the polymerase chain reaction (PCR). The PCR products targeted approximately 2100 nt regions of relatively low G + C content. Comparison of restriction enzyme digests of amplified DNA showed variation useful for strain differentiation. Twelve nonrelated HSV-1 were differentiated from one another. In contrast, specimens epidemiologically related in an outbreak were indistinguishable from each other. Restriction endonuclease analysis of amplified HSV-1 sequences appears to be useful for molecular epidemiology and laboratory quality control to detect possible contamination by PCR products.

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