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J Chromatogr B Biomed Appl. 1994 Dec 2;662(1):61-70.

Automated column liquid chromatographic determination of amoxicillin and cefadroxil in bovine serum and muscle tissue using on-line dialysis for sample preparation.

Author information

1
Department of Analytical Chemistry, Free University, Amsterdam, Netherlands.

Abstract

A fully automated method is described for the determination of amoxicillin and cefadroxil in bovine serum and muscle tissue. The method is based on the on-line combination of dialysis and solid-phase extraction for sample preparation, and column liquid chromatography with ultraviolet detection. In order to enhance the UV detectability of the analytes, post-column addition of 0.1 M sodium hydroxide is performed. The method shows good linearity and repeatability for both analytes in serum as well as in muscle tissue; the limits of detection in these samples are 0.05 microgram/ml and 0.2 microgram/g, respectively. The method has a sample throughput of 30 samples per 24 h.

PMID:
7894694
DOI:
10.1016/0378-4347(94)00377-7
[Indexed for MEDLINE]

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