Send to

Choose Destination
See comment in PubMed Commons below
J Chromatogr B Biomed Appl. 1994 Dec 2;662(1):47-60.

High-performance liquid chromatographic assay of isoflavonoids and coumestrol from human urine.

Author information

Cancer Research Center of Hawaii, Honolulu 96813.


A rapid, sensitive and precise diode-array reversed-phase HPLC method was developed for human urine analysis of the most common dietary isoflavones daidzein, genistein, formononetin and biochanin-A, their mammalian metabolites equol and O-desmethylangolensin, and of coumestrol, another commonly occurring phytoestrogen. Analytes were isolated and concentrated by solid-phase extraction and separated by HPLC followed by identification through retention times and UV scans, and in the case of coumestrol additionally by fluorometric response. This method was applied to monitor changes in urinary excretion of these analytes after challenge with soybeans and was evaluated for precision and recovery of analytes.

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center