Format

Send to

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 1995 Feb 15;207(2):753-60.

Detection and characterization of apoptosis in osteoclasts in vitro.

Author information

1
Department of Orthodontics, Nippon Dental University, School of Dentistry, Tokyo, Japan.

Abstract

Morphological changes of nuclei and DNA fragmentation undergoing cell death were studied using highly purified rabbit osteoclasts in vitro. Approximately fifty percent of dead osteoclasts exhibited morphological changes of nuclei characterized by apoptosis and approximately 70% of osteoclasts with changes of nuclei exhibited DNA fragmentation in our culture system. These changes of nuclei were confirmed as condensation of chromatin through transmission electronmicroscope. Moreover, biochemical characterizations of these changes were compatible with the characterizations of apoptosis in other cell systems. These results demonstrate that part of osteoclasts die by a mode of apoptosis.

PMID:
7864869
DOI:
10.1006/bbrc.1995.1251
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center