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J Chromatogr B Biomed Appl. 1994 Oct 3;660(1):67-73.

Coupled-column high-performance liquid chromatographic method for the determination of four metabolites of polycyclic aromatic hydrocarbons, 1-, 4- and 9-hydroxyphenanthrene and 1-hydroxypyrene, in urine.

Author information

1
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Okologische Chemie, Oberschleissheim, Germany.

Abstract

A coupled-column high-performance liquid chromatographic method applying system-integrated sample processing on a copper phthalocyanine modified solid-phase material, gradient elution and fluorescence detection for the quantification of 1-, 4- and 9-hydroxyphenanthrene as well as for 1-hydroxypyrene in urine is described. The automated chromatographic system tolerates direct and repeated injection of urine samples. Validation results presented indicate quantitative and matrix-independent recoveries, low imprecisions in within-series and between-series analysis in combination with broad linear working ranges (2-400 nmol/l) and low detection limits (< 0.1 nmol/l).

PMID:
7858725
[Indexed for MEDLINE]

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