Format

Send to

Choose Destination
FEBS Lett. 1995 Feb 6;359(1):89-92.

Viral Q beta RNA as a high expression vector for mRNA translation in a cell-free system.

Author information

1
Institute of Protein Research, Russian Academy of Sciences, Moscow Region.

Abstract

Dihydrofolate reductase (DHFR) mRNA was inserted into Q beta phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell-free system resulted in the synthesis of DHFR, which was two orders of magnitude higher than that in the case of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Q beta replicase as compared with its synthesis when the original Q beta RNA was used.

PMID:
7851538
DOI:
10.1016/0014-5793(95)00006-u
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center