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Plasmid. 1994 Sep;32(2):208-11.

Transformation of Bifidobacterium longum with pRM2, a constructed Escherichia coli-B. longum shuttle vector.

Author information

1
Laboratory of Microbial Genetics, University of Bologna, Italy.

Abstract

An Escherichia coli-Bifidobacterium longum shuttle vector, designated pRM2, was constructed by cloning a B. longum plasmid and an enterococcal spectinomycin resistance gene into a commercial E. coli vector. The plasmid was successfully introduced into B. longum cells by electroporation and into E. coli cells by both electroporation and chemical transformation.

PMID:
7846144
DOI:
10.1006/plas.1994.1056
[Indexed for MEDLINE]

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