Purification of lysozyme from human neutrophils, and development of an ELISA for quantification in cells and plasma

K Lollike; L Kjeldsen; H Sengeløv; N Borregaard

Purification of lysozyme from human neutrophils, and development of an ELISA for quantification in cells and plasma

Leukemia. 1995 Jan;9(1):206-9.

Authors

K Lollike¹, L Kjeldsen, H Sengeløv, N Borregaard

Affiliation

¹ Department of Hematology, National University Hospital, Rigshopitalet, Copenhagen, Denmark.

PMID: 7845019

Abstract

Lysozyme was purified from exocytosed granule material from PMA-stimulated human neutrophils by polyethyleneglycol precipitation, cation exchange chromatography and molecular sieve chromatography. Rabbit antibodies were biotinylated and affinity purified on a lysozyme column, for subsequent development of a novel ELISA. This ELISA for lysozyme is sensitive and accurate, and applicable to determination of lysozyme in neutrophils and plasma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Enzyme-Linked Immunosorbent Assay
Humans
Muramidase / blood
Muramidase / immunology
Muramidase / isolation & purification*
Neutrophils / enzymology*
Rabbits

Substances

Muramidase