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Free Radic Res. 1994 Nov-Dec;21(6):409-16.

Antioxidant properties of plastoquinol and other biological prenylquinols in liposomes and solution.

Author information

1
Department of Plant Physiology and Biochemistry, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.

Abstract

Oxidation of biological prenylquinols, like plastoquinol-9 (PQH2-9), ubiquinol-10 (UQH2-10), reduced vitamins K1 (VK1H2) and K2 (VK2H2), alpha-tocopherol quinol (alpha-TQH2) and alpha-tocopherol (alpha-T) was followed by their fluorescence during sonication of egg yolk lecithin/prenylquinol liposomes. The order of magnitude of oxidation of the prenylquinols by free radicals generated during sonication was UQH2-10 > VK2H2 > VK1H2 > alpha-TQH2 > PQH2-9 > alpha-T. It was shown that egg yolk lecithin undergoes degradation even when sonicated briefly under atmosphere of nitrogen and at 0 degree C. A kinetic study of free radical scavenging action of the prenylquinols in solvents of different polarity was performed. The pseudo-first-order rate constants, k, for the reaction of the prenylquinols with 1,1-diphenyl-2-picrylhydrazyl (DPPH) in hexane showed that their scavenging activity changes in the order VK2H2 > VK1H2 > alpha-TQH2 > PQH2-9 > alpha-T > UQH2-10, being the highest in hexane and methanol, whereas in acetone and ethyl acetate the scavenging activity appeared much lower. The reaction rate constants, k, were apparently not dependent on the solvent polarity. The antioxidant activity of the prenylquinols in natural membranes is discussed.

PMID:
7834055
DOI:
10.3109/10715769409056593
[Indexed for MEDLINE]

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