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Microb Pathog. 1994 May;16(5):373-8.

Selective permissiveness of TPA differentiated THP-1 myelomonocytic cells for human cytomegalovirus strains AD169 and Towne.

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Department of Biology, University of Wisconsin-Eau Claire 54702.


Two highly passaged laboratory strains of human cytomegalovirus (HCMV), AD169 and Towne, were tested for their ability to infect and replicate in THP-1 myelomonocytic cells differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA treatment of human THP-1 cells increased the number of cells that expressed HCMV immediate early (IE1) antigen from 0.06% prior to TPA treatment to 12% following cell differentiation. The Towne but not the AD169 strain replicated in differentiated THP-1 cells as determined by HCMV DNA replication and infectious virus production. Major early (HCMVTRL4) mRNA was present in both the abortive and productive THP-1 cell infections.

[Indexed for MEDLINE]

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