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Biochem Biophys Res Commun. 1994 Dec 15;205(2):1291-8.

Molecular construction of Clostridium botulinum type C progenitor toxin and its gene organization.

Author information

1
Department of Bacteriology, Okayama University Medical School, Japan.

Abstract

The 16S progenitor toxin of Clostridium botulinum type C is made up by conjugation of a neurotoxin with nontoxic components designated as nontoxic-nonHA and hemagglutinin (HA). The HA was found to be composed of subcomponents having 53, 33, 22-23, and 17 kDa molecular masses. Since we previously determined the whole nucleotide sequences of the genes for neurotoxin, nontoxic-nonHA, and HA-33, the cloning and nucleotide sequencing of the genes for the remaining HA subcomponents were performed. Two open reading frames coding for 16.7 kDa (HA-17) and 70.6 kDa proteins were identified. The N-terminal amino acid sequences of HA-53 and HA-22-23 indicated that the 70.6 kDa protein is split into 53 and the 22-23 kDa proteins after translation and that the 22-23 kDa protein consists of at least four proteins showing slightly different molecular weights.

PMID:
7802661
DOI:
10.1006/bbrc.1994.2805
[Indexed for MEDLINE]

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