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J Med Virol. 1994 Sep;44(1):54-8.

Rapid detection of virus genome from imported dengue fever and dengue hemorrhagic fever patients by direct polymerase chain reaction.

Author information

1
Department of Virology, Nagasaki University, Japan.

Abstract

Serum specimens from patients with imported dengue fever and dengue hemorrhagic fever were directly subjected to reverse transcription and polymerase chain reaction (RT-PCR) without any RNA purification. The amplified virus genome was detected within 3 hours. The results of PCR corresponded closely to the results of virus isolation using cultured mosquito cells, suggesting that direct RT-PCR procedure greatly facilitates rapid diagnosis of dengue infection.

PMID:
7798887
DOI:
10.1002/jmv.1890440111
[Indexed for MEDLINE]

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