Format

Send to

Choose Destination
Anal Biochem. 1995 Mar 20;226(1):161-6.

A bisulfite method of 5-methylcytosine mapping that minimizes template degradation.

Author information

1
Friedrich Miescher Institut, Basel, Switzerland.

Abstract

The bisulfite method is a highly sensitive approach to 5-methylcytosine mapping that utilizes the capability of the polymerase chain reaction to exponentially amplify DNA. We have observed that the bisulfite reaction results in a significant level of template degradation due to DNA depurination. Furthermore, our data suggest that the DNA fragmentation which occurs limits the sensitivity of the method. We describe a simple solution to limit degradation of the DNA template.

PMID:
7785768
DOI:
10.1006/abio.1995.1204
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center