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Eur J Pharmacol. 1995 Feb 15;288(3):385-8.

5-HT1A receptor-mediated activation of high-affinity GTPase in rat hippocampal membranes.

Author information

1
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.

Abstract

GTP hydrolyzing activity was assayed by measuring the amount of 32P(i) released from 0.3 microM [gamma-32P]GTP in the membranes prepared from rat brain. 5-Hydroxytryptamine (5-HT) stimulated the high-affinity GTPase activity in hippocampus, but not in striatum, in a concentration-dependent manner with an EC50 value of 18 nM and maximal percent stimulation of 13.9%. This response was mimicked by (+/-)-8-hydroxy-2-(di-n-propylamino)tetralin [(+/-)-8-OH-DPAT], but not by (+/-)-1-(2,5-dimethoxy-4-iodophyenyl)-2-aminopropane [(+/-)-DOI]. These results suggest that 5-HT-stimulated high-affinity GTPase activity of the GTP-binding protein(s) is mediated via 5-HT1A receptor subtype in the rat hippocampus.

PMID:
7774684
DOI:
10.1016/0922-4106(95)90053-5
[Indexed for MEDLINE]

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