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Cell. 1995 Jun 2;81(5):667-76.

ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome.

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1
Cold Spring Harbor Laboratory, New York 11724, USA.

Abstract

The origin recognition complex (ORC) binds replicators in the yeast S. cerevisiae in a manner consistent with it being an initiator protein for DNA replication. Two-dimensional (2D) gel techniques were used to examine directly initiation of chromosomal DNA replication in temperature-sensitive orc mutants. Unlike in wild-type cells, in orc2-1 and orc5-1 mutant cells, only a subset of replicators formed active origins of DNA replication at the permissive temperature. At the restrictive temperature, the number of active replicators was diminished further. Using a genetic screen, CDC6 was identified as a multicopy suppressor of orc5-1. 2D gel and biochemical analyses demonstrated that Cdc6p interacted functionally and physically with ORC. We suggest that ORC and Cdc6p form a prereplication complex at individual replicators and therefore cooperate to determine the frequency of initiation of DNA replication in the genome.

PMID:
7774008
DOI:
10.1016/0092-8674(95)90528-6
[Indexed for MEDLINE]
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