Abstract
The Mxi1 protein negatively regulates Myc oncoprotein activity and thus potentially serves a tumour suppressor function. MXI1 maps to chromosome 10q24-q25, a region that is deleted in some cases of prostate cancer. We have detected mutations in the retained MXI1 alleles in four primary prostate tumours with 10q24-q25 deletions. Two tumours contained inactivating mutations, whereas two others contained the identical missense mutation. Fluorescence in situ hybridization also demonstrated loss of one MXI1 allele in an additional tumour lacking chromosome 10 abnormalities. MXI1 thus displays allelic loss and mutation in some cases of prostate cancer that may contribute to the pathogenesis or neoplastic evolution of this common malignancy.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Base Sequence
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Basic Helix-Loop-Helix Transcription Factors
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Chromosome Aberrations
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Chromosome Mapping
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Chromosomes, Human, Pair 10
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DNA Primers / genetics
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DNA, Neoplasm / genetics
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DNA, Neoplasm / metabolism
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Genes, Tumor Suppressor*
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Humans
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In Situ Hybridization, Fluorescence
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Male
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Molecular Sequence Data
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Mutation*
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Prostatic Neoplasms / genetics*
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Prostatic Neoplasms / metabolism
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Sequence Deletion
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Transcription Factors*
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Tumor Suppressor Proteins
Substances
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Basic Helix-Loop-Helix Transcription Factors
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DNA Primers
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DNA, Neoplasm
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DNA-Binding Proteins
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MXI1 protein, human
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Transcription Factors
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Tumor Suppressor Proteins